Epidemic Diarrheal Disease of Suckling Mice Iv. Cytoplasmic Inclusion Bodies in Intestinal Epithelium In" Relation to the Disease*~ by Alwin M. Pappenheimer, M.d.,§ and F. Sargent Cheever, M.d

In a previous report (1), Pappenheimer and Enders described the occurrence of intranuclear inclusions in a large proportion of suckling mice with diarrhea (Fig. 1). The original stock in which the spontaneous disease had been prevalent for several years was virtually destroyed during the summer of 1945. Since then, new stock from several sources has developed spontaneous diarrhea, indistinguishable, as far as obvious signs are concerned, from the disease as it appeared in the original stock. The epidemiology of the disease as now prevalent is described in the preceding paper (2). In no instance have we succeeded in demonstrating intranuclear inclusions of the type previously described. However, many of the mice have had cytoplasmic inclusions in the epithelial cells of the small intestine. In this study, evidence is presented bearing on the possible relation of these cytoplasmic inclusion bodies to the diarrheal disease as it has occurred in our colony subsequent to the introduction of new strains.